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Wednesday, August 12, 2020 | History

4 edition of Characterization of Agrobacterium tumefaciens adsorption to potato tissue found in the catalog.

Characterization of Agrobacterium tumefaciens adsorption to potato tissue

by Daniel Albert Kluepfel

  • 100 Want to read
  • 5 Currently reading

Published .
Written in English

    Subjects:
  • Agrobacterium tumefaciens.,
  • Potatoes -- Diseases and pests.,
  • Phytopathogenic bacteria.

  • Edition Notes

    Statementby Daniel Albert Kluepfel
    The Physical Object
    Paginationvi, 134 leaves :
    Number of Pages134
    ID Numbers
    Open LibraryOL24135235M
    OCLC/WorldCa11494285

    The human population has reached 7 billion by and is estimated to exceed 10 billion by the end of As such, crops which are the main food source must be produced at a higher pace in order to cater in tandem with the food demand. In the past, traditional plant breeders practice classical breeding techniques to propagate plants with desirable traits.   While Agrobacterium-mediated transformation (AMT) remains the most widely used technique for gene transfer in plants, interest exists for the use of non-Agrobacterium gene delivery systems due to freedom-to-operate issues that remain with AMT across several jurisdictions. In addition, the plant pathogenic mode of action of Agrobacterium tumefaciens significantly increases the costs .

    A grobacterium tumefaciens is aerobic, Gram negative, rod shaped and motile bacterium that belongs to the family Rhizobiaceae. These bacteria do not form endospores (Davoodiand Hajivand, ).A. tumefaciens enters plant tissues through wounds and causes infection producing tumours on a broad range of plants including many dicots, few monocots and some gymnosperms. Abstract: A method for increasing efficiency of germplasm screening for transformability may include providing a plurality of lines of plant target tissue to be transformed, characterizing each of the lines to provide characterization data, the characterization data comprises DNA or nucleic acid delivery technique response data and tissue culture response data, eliminating one or more of the.

    Agrobacterium-mediated transformation of non-plant organisms. In Agrobacterium: From Biology to Biotechnology (Tzfira T and Citovsky V eds.), pp. , Springer, New York, Windels P, De Buck S and Depicker A. Agrobacterium tumefaciens-mediated transformation: pattern of T-DNA integration into the host genome.   This is how Agrobacterium tumefaciens could change plant cells into tumor cells. Significantly, this is also the reason as to why gene exchange could be made possible between unrelated plant species. This opened a window for using the natural genetic engineering capabilities of the Agrobacterium bacteria as a tool to make transgenic plants.


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Characterization of Agrobacterium tumefaciens adsorption to potato tissue by Daniel Albert Kluepfel Download PDF EPUB FB2

TABLEOFCONTENTS PAGE ACKNOWLEDGEMENTS ii ABSTRACT v CHAPTERONEINTRODUCTION I CHAPTERTWOLITERATUREREVIEW 3 GeneralAdsorptionChemistry 3 AdsorptionofBacterialInvadors 8. the adsorption isotherm at these bacterial concentrations is described by the linear relationship Cb = kC5, where Cb is the number of bound bacteria, C, is the number of free bacteria, and kis a constant (4, 7).

Log-transformed values TABLE 1. Kinetics ofthe adsorption ofA. tumefaciens B6to potato tuber tissue Adsorption No. ofbacteria ( Cited by: 3.

Abstract. Potato tuber disks were submerged in suspensions containing 10 1 to 10 9 cells of Agrobacterium tumefaciens B6 per ml. After 60 min, the disks were rinsed and homogenized, and portions of the homogenates were plated to measure the number of adsorbed by: 4.

Adsorption of Agrobacterium tumefaciens to Susceptible Potato Tissues: a Physisorption Process Article (PDF Available) in Applied and Environmental Characterization of Agrobacterium tumefaciens adsorption to potato tissue book 51(5). Adsorption of Agrobacterium tumefaciens to resistant and susceptible plant tissues and to nonbiological surfaces DANIEL A.

KLUEPFULt and STEVEN G. PUEPPKEt Department ifPlantPathology, University ifFlorida, Gainesville, FloridaU,S,A. (AcceptedJor publication May ) We labelled cells of tumorigenic and nontumorigenic strains of Agrobacterium tumefaciens Author: Daniel A. Kluepful, Steven G. Pueppke. Potato tuber moth (Phthorimaea operculella) is a major insect pest of potatoes during field cultivation and post harvest storage, causing high annual losses in potato production Bacillus thuringiensis gene (cry1Ia1) was introduced into the South African potato cultivar Mnandi with Agrobacterium-mediated Agrobacterium tumefaciens strain LBA.

Agrobacterium tumefaciens is able to generate crown galls in the infected tissue of many plant species by the action of its tumour-inducing (Ti) plasmid.

The plasmid contains a T-DNA region with a set of genes that will be integrated in the plant genome and induce tissue dedifferentiation 1, 2.

In vitro potato explants were infected with Agrobacterium LBA, cultured on the pre-culture medium with 50 μM acetosyr-ingone, co-cultivated on pre-culture media for two days, and then.

Song Guo-Q, Honda H, Yamaguchi Ken-I. Efficient Agrobacterium tumefaciens mediated transformation of sweet potato (Ipomoea batatas (L.) Lam.) from stem explants using a two-step kanamycin-hygromycin selection method.

In Vitro Cellular and Developmental Biology. ; Michael PS. Sweet potato [Ipomoea batatas (L.)] genetic transformation. Agrobacterium rhizogenes (or Rhizobium rhizogenes) is able to transform plant genomes and induce the production of hairy roots. We describe the use of A. rhizogenes in tomato (Solanum spp.) to rapidly assess gene expression and function.

Gene expression of reporters is indistinguishable in plants transformed by Agrobacterium tumefaciens as compared with A. rhizogenes. Major steps of the Agrobacterium tumefaciens-mediated plant transformation process. (1) Attachment of A. tumefaciens to the plant cells.(2) Sensing plant signals by A.

tumefaciens and regulation of virulence genes in bacteria following transduction of the sensed signals.(3) Generation and transport of T-DNA and virulence proteins from the bacterial cells into plant cells. Agrobacterium is a genus of Gram-negative bacteria established by H.

Conn that uses horizontal gene transfer to cause tumors in plants. Agrobacterium tumefaciens is the most commonly studied species in this genus. Agrobacterium is well known for its ability to transfer DNA between itself and plants, and for this reason it has become an important tool for genetic engineering.

Agrobacterium tumefaciens has been utilized for both transient and stable transformations of plants. These transformation methods have been used in fields such as breeding GM crops, protein production in plant cells, and the functional analysis of genes.

However, some plants have significantly lower transient gene transfer and stable transformation rates, creating a technical barrier that. type strian of A. tumefaciens (ATCCT). Supported by statistical analysis of the number of induced tumors in potato disc strain AtTp from Tagetes patula was identifi ed as more virulent than the other isolates.

Key words Agrobacterium tumefaciens, wild type, virulence, phytopathogenicity, antibiotic sen-sitivity and plasmid DNA. Characterization of three Agrobacterium tumefaciens avirulent mutants with chromosomal mutations that affect induction of vir genes. J Metts, J West, S H Doares, and A G Matthysse Department of Biology, University of North Carolina, Chapel Hill Agrobacterium tumefaciens, Almond, pathogenecity tests, biochemical characterization.

Introduction: Crown gall caused by (Smith and Agrobacterium tumefaciens Townsend) Conn., is widespread and has worldwide distribution (Matthow et al., ).

It is considered to be a disease of great economic importance in. Abstract. Potato is considered the fourth most important food crop in the world, and the most important non-cereal crop. Potato is transformed using Agrobacterium tumefaciens with relative ease. Several improvements have been made in the last 20 years with respect to tissue culture, transformation, and regeneration of potato.

Agrobacterium tumefaciens (updated scientific name Rhizobium radiobacter, synonym Agrobacterium radiobacter) is the causal agent of crown gall disease (the formation of tumours) in over species of is a rod-shaped, Gram-negative soil bacterium. Symptoms are caused by the insertion of a small segment of DNA (known as the T-DNA, for 'transfer DNA', not to be confused with tRNA.

Characterization of an Agrobacterium tumefaciens D-Psicose 3-Epimerase That Converts D-Fructose to D-Psicose Hye-Jung Kim,† Eun-Kyung Hyun,† Yeong-Su Kim,† Yong-Joo Lee,† and Deok-Kun Oh* Department of Bioscience and Biotechnology, Sejong University, SeoulSouth Korea Received 12 September /Accepted 5 November   Mahzad Sharifahmadian, Christian Baron, Type IV Secretion in Agrobacterium tumefaciens and Development of Specific Inhibitors, Type IV Secretion in Gram-Negative and Gram-Positive Bacteria, /_7, (), ().

The laboratory Agrobacterium tumefaciens strain C58C1 was the best strain for use in plant species that did not elicit a necrotic response to A. tumefaciens. A wild A. tumefaciens strain, 1D, was identified that provided high levels of transient expression in solanaceous plants without background necrosis, enabling routine transient assays.Abstract.

The soil phytopathogen Agrobacterium tumefaciens induces tumors, known as crown galls, mainly on dicotyledonous plants. Such tumors are generated by a complex, multi-step transformation process.

Agrobacterium has been routinely utilized for the transfer of genes to dicotyledonous plants, and monocotyledonous plants, including important cereals, were thought until recently to be. Book Description. Alternating between topic discussions and hands-on laboratory experiments that range from the in vitro flowering of roses to tissue culture of ferns, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, addresses the most current principles and methods in plant tissue culture research.

The editors use the expertise of some of the top researchers and.